Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes

Agus Hery Susanto, Suhestri Suryaningsih

Abstract


Penjalin water reservoir in Brebes Regency, Central Java, is one of the habitats of the sand goby. A study on its genetic diversity using approaches of isozyme analysis was needed to support domestication of the fish in this area. This study was aimed at the biochemical-genetic variation of sand goby population in Penjalin water reservoir based on esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), aspartate amino-transferase (AAT), and acid phosphatase (ACP) polymorphisms. Visualization of the isozymes was carried out employing horizontal electrophoretic technique with potato starch gel and buffer system of L-histidin monohydrate. Of the five isozymes, ACP was not well-visualized in all samples tested while the remaining four showed no polymorphisms. It could be concluded that there is no biochemical-genetic variation of sand goby population in Penjalin water reservoir based on isozymes of EST, PER, MDH, and AAT.

Keywords


sand goby, isozyme, biochemical-genetic variation, horizontal electrophoresis

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DOI: https://doi.org/10.24002/biota.v11i3.2539

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