Analisis Filogenetik Burung Maleo (Macrocephalon maleo) Berdasarkan Sekuen Intron Satu Gen Rhodopsin (RDP1) Nukleus

I Made Budiarsa, I Wayan Tunas Artama, Langkah Sembiring, Jesmandt Situmorang

Abstract


The phylogenetic relationships of the maleo (Macrocephalon maleo) were analyzed based on the
first intron of rhodopsin nuclear gene sequence data obtained from 15 individuals, along with
those of 22 individuals taken from GenBank. The phylogenetic trees were reconstructed by
Neighbor-Joining (NJ) method. Results indicated that 956 bp of RDP1 sequence, 414 (43.4%)
sites were variable and 317 (33.2%) sites were phylogenetically-informative. The base
composition for all species analyzed in this research were as follows: T 25.3%, C 26.3%, A
18.5%, and G 29.9%. Analysis of RDP1 sequence produced trees that were remarkably well
resolved and had topologies at the marga level. The phylogenetic analysis showed that maleo
was monophyly of Macrocephalon and closely related to Aepypodius, Talegalla, Leipoa and
Alectura.


Keywords


Macrocephalon maleo, phylogenetic, genetic diversity, RDP1

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DOI: https://doi.org/10.24002/biota.v15i2.2693

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