Dekolorisasi Reactive Red oleh Konsorsium Bacillus velezensis IBLTT_1 dan Bacillus cereus IBLTT_5
DOI:
https://doi.org/10.24002/biota.v11i1.11205Keywords:
Pewarna azo, efisiensi dekolorisasi, inokulum, kultur campur, pengolahan air limbahAbstract
Perkembangan industri tekstil di Indonesia telah berkontribusi pada polusi lingkungan, yaitu melalui pembuangan air limbah yang mengandung pewarna. Diperkirakan sekitar 10-15% pewarna tekstil yang digunakan dalam proses pewarnaan dilepaskan ke dalamke dalam air bilasan dan selanjutnya dibuang ke lingkungan perairan. Air limbah yang mengandung pewarna tekstil harus diolah karena dapat mencemari lingkungan. Reactive Red merupakan salah satu pewarna azo yang banyak digunakan dalam industri tekstil. Salah satu pendekatan untuk mengoptimalkan proses degradasi pewarna azo adalah dengan memutus ikatan azo menggunakan kultur campuran bakteri. Penelitian ini bertujuan untuk menentukan potensi kultur tunggal dan campuran isolat Bacillus velezensis IBLTT_1 dan Bacillus cereus IBLTT_5 dalam mendekolorisasi Reactive Red. Kedua isolat bakteri tersebut diinokulasi secara tunggal dan campur ke dalam media yang mengandung Reactive Red dengan empat perlakuan konsentrasi inokula yaitu 0%, 2%, 4%, dan 8% dari volume kerja. Dekolorisasi tertinggi (76,80%) ditemukan pada kultur campuran dengan konsentrasi 8%, dimana kedua spesies bakteri ditambahkan dalam perbandingan 1:1. Dekolorisasi yang signifikan pada kultur campuran kemungkinan disebabkan oleh aksi sinergis antara dua spesies bakteri yang diinokulasikan secara bersamaan.
References
Al-Garni, S. M., Ghanem, K. M., Kabli, S. A., & Biag, A. K. (2013). Decolorization of Crystal Violet by mono and mixed bacterial culture techniques using optimized culture conditions. Polish Journal of Environmental Studies, 22(5), 1297–1306.
Alzain, H., Kalimugogo, V., & Hussein, K. (2023). A review of bacterial degradation of Azo Dyes. International Journal of Research and Review, 10(6), 443-462. https://doi.org/10.52403/ijrr.20230657.
Chaurasia, P., Jasuja, N. D., & Kumar, S. (2024). Isolation and characterization of bacterial consortia for bioremediation of textile dye effluent. Biopesticides International, 20(1), 151–157. https://doi.org/10.59467/BI.2024.20.151.
Hannan, I. A., Eka Witrie, S., & Prasetya Adi, N. (2024). Dampak pencemaran air akibat limbah industri batik printing di kecamatan pekalongan utara terhadap kualitas air sungai. Gudang Jurnal Multidisiplin Ilmu, 2(8), 34–42. https://doi.org/10.59435/gjmi.v2i8.774.
Garzóón-Zúñga MA, Sandoval-Villasana AM, Moeller-Chávez GE. (2011). Decolorization of the AO24 azo dye and reduction of toxicity and genotoxicity in trickling biofilters. Water Environment Research, 83(2), 107-15. https://doi.org/10.2175/106143010x12780288627977.
Kamal, I. M., Abdeltawab, N. F., Ragab, Y. M., Farag, M. A., & Ramadan, M. A. (2022). Biodegradation, decolorization, and detoxification of di-azo dye Direct Red 81 by halotolerant, alkali-thermo-tolerant bacterial mixed cultures. Microorganisms, 10(5), 994. https://doi.org/10.3390/microorganisms10050994.
Kara, P. I., Meitiniarti, V. I., & Timotius, K. H. (2019). Pertumbuhan Enterococcus faecalis ID 6017 dan kemampuan dekolorisasi beberapa konsentrasi Orange II dalam sistem sinambung. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 13(2), 55–61. https://doi.org/10.24002/biota.v13i2.2671.
Karpova, E. E., Kunz, G. I., & Garner, M. B. (2021). Sustainability in Textile Dyeing: Recent Developments. In Muthu, S., Gardetti, M. (Ed.), Sustainable Textiles: Production, Processing, Manufacturing & Chemistry. Springer. https://doi.org/10.1007/978-3-030-38545-3_2
Manzoor, J., & Sharma, M. (2020). Impact of Textile Dyes on Human Health and Environment. In Wani, K.A., Jangid, N.K., & Bhat, A.R. (Ed.), Impact of Textile Dyes on Public Health and the Environment. IGI Global Scientific Publishing. https://doi.org/10.4018/978-1-7998-0311-9.ch008
Meitiniarti, V. I., & Nugroho, R. A. (2022). Ragam Bakteri Pendekolorisasi Pewarna Sumifix Blue dan Reactive Red 2. Seminar Nasional VII Prodi Pendidikan Biologi, 695-700.
Meitiniarti, V. I., Timotius, K. H., & Sugiharto, E. (2012). Decolorization of Orange II by mixed culture of Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016. Microbiology Indonesia, 6(3), 17-23
Mustaha, G., Zahid, T. M., Kurade, B. M., Alvi, A., Ullah, F., Yadaf, N., Park, H., Khan, A. M., & Jeon, H. B. (2024). Microalgal and activated sludge processing for biodegradation of textile dyes. Environmental Pollution, 349(6), 123902. https://doi.org/10.1016/j.envpol.2024.123902
Nasrin, T., Saha, A. K., Mohanta, M. K., Chaity, A. S., Alam, M. J., Shawon, M. M. H., & Haque, M. F. (2022). Reduction of toxic effects of textile dye, Basic Red-18 on tilapia fish by bioremediation with a novel bacterium, Mangrovibacter yixingensis strain aks2 isolated from textile wastewater. Annual Research & Review in Biology, 37(11), 12–29. DOI:10.9734/arrb/2022/v37i1130544
Nurani, I. K. (2019). Karakterisasi Potensi Isolat Bakteri IBLTT-1 dan IBLTT-2 untuk Mendegradasi Pewarna Reactive Red II [Bachelor's Thesis]. Universitas Kristen Satya Wacana.
Patel Jay, N., Vaishnav Darshit, K., Joshi Pavan, R., & Tipre Devayani, R. (2023). Bacterial enzymes for azo dye degradation: An insight. Research Journal of Chemistry and Environment 27(4), 135–148. https://doi.org/10.25303/2704rjce1350148
Permatasari, I., Nugroho, R. A., & Meitiniarti, V. I. (2018). Dekolorisasi pewarna tekstil Sumifix Blue dan Reactive Red 2 oleh bakteri yang diisolasi dari limbah industri tekstil. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(1), 20-26. https://doi.org/10.29122/jbbi.v5i1.2757
Rani, C., Jana, A. K., & Bansal, A. (2011). Studies on the biodegradation of azo dyes by white rot fungi Daedalea flavida in the absence of external carbon source. 2nd International Conference on Environmental Science and Technology, 6, 147–150.
Rohyami, Y., Ratri, H. P. I., & Wihyarti, W. (2018). Validasi metode penentuan Rhodamin B dalam contoh saos secara spektrofotometri UV-Vis dengan dua variasi pelarut. IJCA (Indonesian Journal of Chemical Analysis), 1(01), 20–28. https://doi.org/10.20885/ijca.vol1.iss1.art3
Sarkar, S., Banerjee, A., Halder, U., Biswas, R., & Bandopadhyay, R. (2017). Degradation of synthetic azo dyes of textile industry: A sustainable approach using microbial enzymes. Water Conservation Science and Engineering 2(4), 121–131. https://doi.org/10.1007/s41101-017-0031-5
Shah, M. P., & Bera, S. P. (2021). Microbial treatment of textile dye Reactive Red 3 by a newly developed bacterial consortium. Nanotechnology for Environmental Engineering, 6(62). https://doi.org/10.1007/s41204-021-00156-7
Singh, S. S., Salem, R. D., & Sani, K. R. (2021). Spectroscopy, microscopy, and other techniques for characterization of bacterial nanocellulose and comparison with plant-derived nanocellulose. In Microbial and Natural Macromolecules. Academic Press. https://doi.org/10.1016/B978-0-12-820084-1.00018-1
Tomilayo, R. B., Sani, A., & Sule, I. O. (2019). Potentials of mixed cultures of bacteria in the biodegradation of crude oil. Ife Journal of Science, 21(3), 49-57. https://doi.org/10.4314/ijs.v21i3.5
Unnikrishnan, S., Khan, M. H., & Ramalingam, K. (2018). Dye-tolerant marine Acinetobacter baumannii-mediated biodegradation of Reactive Red. Water Science and Engineering, 11(4), 265–275. https://doi.org/10.1016/j.wse.2018.08.001.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Erinna Azalia Fitri, Vincentia Irene Meitiniarti, Rully Adi Nugroho
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati agree to the following terms:
- Authors retain copyright and grant the Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati right of first publication. Licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, and as long as Author is not used for commercial purposes.
