Phosphate-Solubilizing Bacteria with Solid Carrier Material in The Cultivation of Sweet Corn
DOI:
https://doi.org/10.24002/biota.v11i1.12477Keywords:
Husk charcoal, phosphate solubilizing bacteria, shrimp shells, sweet cornAbstract
The limited availability of fertile land is a strong reason to utilize marginal land. Marginal land has potential for agricultural development because it has relatively high total phosphorus, but this phosphorus is in a form that is not available to plants. The use of phosphate-solubilizing bacteria (PSB) can be an alternative to change this form. The effectiveness of PSB activity in altering this form can be enhanced through carriers that are able to maintain their viability and activity in the soil. This study investigates the use of marginal land treated with phosphate solubilizing bacteria (PSB) incorporated with carrier materials to addressing the demand for sweet corn. PSB application followed a Randomized Block Design (RBD) involving the treatments as follows: control (T0), 150 kg Super Phosphate-36 (SP-36) per hectare (T1), PSB B5(6) + shrimp shells + 75 kg SP-36 per hectare (T2), PSB B1(17) + shrimp shells + 75 kg SP-36 per hectare (T3), PSB B5(6) + husk charcoal + 75 kg SP-36 per hectare (T4) and PSB B1(17) + husk charcoal + 75 kg SP-36 per hectare (T5). The results yield the optimum outcome associated with T2 by plant height (±70.25 cm), number of leaves (±8.97 pieces) and roots (±41 cm), root length (±31.10 cm) and volume (±14.05 ml), plant fresh (±53.72 gr) and dry weight (±28.01 gr), cob weight with husk (± 23.68) and without husk (±14.67 gr), cob length with husk (±12.88 cm) and without husk (±7.38 cm), cob diameter with husk (±22.76 cm) and without husk (±16.67 cm). T2 can reduce the use of inorganic phosphorus fertilizer up to 50% and increase production by approximately 7 times better than control.
References
Abirami, S., Gnanamuthu, G., & Nagarajan, D. (2022). Bioconversion of shrimp shell waste into compost preparation and its plant growth study. Indian Journal of Agricultural Research, 56(5), 588–593. https://doi.org/10.18805/IJARe.A-5214
Aditama, W., Zulfikar, Z., & Sofia, S. (2024). Effects of ceramic membrane composed of clay and a mixture of rice husks, sawdust, coconut shell charcoal, and coffee grounds on reducing total coliforms in well water. International Journal of Environmental Health Engineering, 13(1), 26–36. https://doi.org/10.4103/ijehe.ijehe_2_24
Akhadi, D. H., Suyanti, S., Herlina, S., Amandita, F. Y., Suryati, T., Andriyani, R., Gafur, N. A., Apriyana, A. Y., Zulaikha, S., & Hidayati, N. (2024). Mercury absorption using rice husk charcoal inoculated with five resistant bacteria. IOP Conference Series: Earth and Environmental Science, 1388(1), 012017. https://doi.org/10.1088/1755-1315/1388/1/012017
Anjum, N. A., Masood, A., Umar, S., & Khan, N. A. (2024). Phosphorus in Soils and Plants. IntechOpen.
Arif, M. S., Shahzad, S. M., Yasmeen, T., Riaz, M., Ashraf, M., Ashraf, M. A., Mubarik, M. S., & Kausar, R. (2017). Improving plant phosphorus (P) acquisition by phosphate-solubilizing bacteria. In Essential Plant Nutrients (pp. 513–556). Springer. https://doi.org/10.1007/978-3-319-58841-4_21
Azam, H. M., Alam, S. T., Hasan, M., Yameogo, D. D. S., Kannan, A. D., Rahman, A., & Kwon, M. J. (2019). Phosphorous in the environment: Characteristics with distribution and effects, removal mechanisms, treatment technologies, and factors affecting recovery as minerals in natural and engineered systems. Environmental Science and Pollution Research, 26(20), 20183–20207. https://doi.org/10.1007/s11356-019-04732-y
Barrow, N. J., & Hartemink, A. E. (2023). The effects of pH on nutrient availability depend on both soils and plants. Plant and Soil, 487(1–2), 21–37. https://doi.org/10.1007/s11104-023-05960-5
Beauchamp, E. G., Binkley, D., Buresh, R. J., De Datta, S. K., Hart, S. C., McBride, M. B., Paul, J. W., Trevors, J. T., & van Wambeke, A. (2012). Advances in Soil Science. Springer Science & Business Media.
Billah, M., Khan, M., Bano, A., Hassan, T. U., Munir, A., & Gurmani, A. R. (2019). Phosphorus and phosphate solubilizing bacteria: Keys for sustainable agriculture. Geomicrobiology Journal, 36(10), 904–916. https://doi.org/10.1080/01490451.2019.1654043
Chen, W., Yang, F., Zhang, L., & Wang, J. (2016). Organic acid secretion and phosphate solubilizing efficiency of Pseudomonas sp. PSB12: Effects of phosphorus forms and carbon sources. Geomicrobiology Journal, 33(10), 870–877. https://doi.org/10.1080/01490451.2015.1123329
Chen, Y., Han, W., Tang, L., Tang, Z., & Fang, J. (2013). Leaf nitrogen and phosphorus concentrations of woody plants differ in responses to climate, soil and plant growth form. Ecography, 36(2), 178–184. https://doi.org/10.1111/j.1600-0587.2011.06833.x
Elhaissoufi, W., Ghoulam, C., Barakat, A., Zeroual, Y., & Bargaz, A. (2022). Phosphate bacterial solubilization: A key rhizosphere driving force enabling higher P use efficiency and crop productivity. Journal of Advanced Research 38, 13–28. https://doi.org/10.1016/j.jare.2021.08.014
Etesami, H. (2020). Enhanced Phosphorus Fertilizer Use Efficiency with Microorganisms. In Nutrient Dynamics for Sustainable Crop Production (pp. 215–245). Springer. https://doi.org/10.1007/978-981-13-8660-2_8
Iftikhar, A., Aijaz, N., Farooq, R., Aslam, S., Zeeshan, A., Munir, M., Irfan, M., Mehmood, T., Atif, M., & Ali, M. (2023). Beneficial role of phosphate solubilizing bacteria (PSB) in enhancing soil fertility through a variety of actions on plants growth and ecological perspective: An updated review. Journal of Xi’an Shiyou University, Natural Science Edition, 19(9), 520–547.
Indriani, M., Hadi, P., Rachmawatie, S. J., & Widyatama, A. (2025). Optimasi hasil tanaman brokoli (Brassica oleracea L. Var. Italica) di dataran rendah dengan pemangkasan tunas lateral dan macam pupuk organik cair. Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian, 8(1), 1-6.
Kahura, M. W., Min, H.-G., Kim, M.-S., & Kim, J.-G. (2018). Assessing phosphorus availability in a high pH, biochar amended soil under inorganic and organic fertilization. Ecology and Resilient Infrastructure, 5(1), 11–18. https://doi.org/10.17820/eri.2018.5.1.011
Karthika, K. S., Rashmi, I., & Parvathi, M. S. (2018). Biological functions, uptake and transport of essential nutrients in relation to plant growth. In Plant Nutrients and Abiotic Stress Tolerance (pp. 1–49). Springer https://doi.org/10.1007/978-981-10-9044-8_1
Kaur, C., Selvakumar, G., & Ganeshamurthy, A. N. (2016). Organic acids in the rhizosphere: Their role in phosphate dissolution. In Microbial Inoculants in Sustainable Agricultural Productivity. Springer https://doi.org/10.1007/978-81-322-2644-4_11
Khan, F., Siddique, A. B., Shabala, S., Zhou, M., & Zhao, C. (2023). Phosphorus plays key roles in regulating plants’ physiological responses to abiotic stresses. Plants, 12(15), 2861. https://doi.org/10.3390/plants12152861
Lazo, D. E., Dyer, L. G., & Alorro, R. D. (2017). Silicate, phosphate and carbonate mineral dissolution behaviour in the presence of organic acids: A review. Minerals Engineering, 100, 115–123. https://doi.org/10.1016/j.mineng.2016.10.013
Mansyur, N. I., Pudjiwati, E. H., & Murtilaksono, A. (2021). Pupuk dan Pemupukan. Syiah Kuala University Press, Indonesia.
Pal, K., Rakshit, S., Mondal, K. C., & Halder, S. K. (2021). Microbial decomposition of crustacean shell for production of bioactive metabolites and study of its fertilizing potential. Environmental Science and Pollution Research, 28(42), 58915–58928. https://doi.org/10.1007/s11356-021-13109-z
Pudjiwati, E. H., Zahara, S., & Sartika, D. (2019). Isolasi dan karakterisasi rhizobakteri yang berpotensi sebagai agen pemacu pertumbuhan tanaman. Jurnal Borneo Saintek, 2(2), 01–10. https://doi.org/10.35334/borneo_saintek.v2i2.1084
Rahman, M. M., & Maniruzzaman, M. (2023). A new route of production of the meso-porous chitosan with well-organized honeycomb surface microstructure from shrimp waste without destroying the original structure of native shells: Extraction, modification and characterization study. Results in Engineering, 19, 101362. https://doi.org/10.1016/j.rineng.2023.101362
Rawat, P., Das, S., Shankhdhar, D., & Shankhdhar, S. C. (2021). Phosphate solubilizing microorganisms: mechanism and their role in phosphate solubilization and uptake. Journal of Soil Science and Plant Nutrition, 21(1), 49–68. https://doi.org/10.1007/s42729-020-00342-7
Rodrı́guez, H., & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17(4–5), 319–339. https://doi.org/10.1016/S0734-9750(99)00014-2
Safitri, A., & Susilowati, L. E. (2025). Dampak perubahan iklim terhadap komunitas mikroba tanah dan implikasinya pada pertumbuhan dan produksi tanaman. Jurnal Ilmiah Mahasiswa Agrokomplek, 4(3), 804–813. https://doi.org/10.29303/fwpexd49
Sahu, P. K., & Brahmaprakash, G. P. (2016). Formulations of Biofertilizers – approaches and advances. In Microbial Inoculants in Sustainable Agricultural Productivity. Springer. https://doi.org/10.1007/978-81-322-2644-4_12
Singh, C., Tiwari, S., Gupta, V. K., & Singh, J. S. (2018). The effect of rice husk biochar on soil nutrient status, microbial biomass and paddy productivity of nutrient poor agriculture soils. Catena, 171, 485–493. https://doi.org/10.1016/j.catena.2018.07.042
Thunshirn, P., Wenzel, W. W., & Pfeifer, C. (2022). Pore characteristics of hydrochars and their role as a vector for soil bacteria: A critical review of engineering options. Critical Reviews in Environmental Science and Technology, 52(23), 4147–4171. https://doi.org/10.1080/10643389.2021.1974256
Vilar Junior, J. C., Ribeaux, D. R., Alves Da Silva, C. A., & De Campos-Takaki, G. M. (2016). Physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells. International Journal of Microbiology, 2016, 1–7. https://doi.org/10.1155/2016/5127515
Wang, Z., Zhang, H., Liu, L., Li, S., Xie, J., Xue, X., & Jiang, Y. (2022). Screening of phosphate-solubilizing bacteria and their abilities of phosphorus solubilization and wheat growth promotion. BMC Microbiology, 22(1), 296. https://doi.org/10.1186/s12866-022-02715-7
Xu, J., Liu, S., Song, S., Guo, H., Tang, J., Yong, J. W., Ma, Y., & Chen, X. (2018). Arbuscular mycorrhizal fungi influence decomposition and the associated soil microbial community under different soil phosphorus availability. Soil Biology and Biochemistry, 120(6), 181–190. https://doi.org/10.1016/j.soilbio.2018.02.010
Zhang, S., Zheng, Q., Noll, L., Hu, Y., & Wanek, W. (2019). Environmental effects on soil microbial nitrogen use efficiency are controlled by allocation of organic nitrogen to microbial growth and regulate gross N mineralization. Soil Biology and Biochemistry, 135, 304–315. https://doi.org/10.1016/j.soilbio.2019.05.019
Zhu, Y., Xing, Y., Li, Y., Jia, J., Ying, Y., & Shi, W. (2024). The role of phosphate-solubilizing microbial interactions in phosphorus activation and utilization in plant–soil systems: A review. Plants, 13(19), 2686. https://doi.org/10.3390/plants13192686
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