Trichoderma-Based Biocontrol Strategies Against Fusarium Wilt: A Bibliometric Analysis and literature Review

Authors

  • Anton Meilus Putra Department of Plant Protection, Faculty of Agriculture, Universitas Sriwijaya, Indralaya

DOI:

https://doi.org/10.24002/biota.v11i1.12938

Keywords:

Bibliometric analysis, biocontrol agents, bio stimulant, biotic stress, plant defence

Abstract

Fusarium wilt is one of the most destructive soil-borne diseases threatening global food security. For decades, its control has relied heavily on synthetic chemical fungicides; however, their extensive use has led to pathogen resistance, environmental degradation, and risks to human health. Trichoderma has been widely investigated as a potential biological control agent due to its diverse mechanisms of action. This study aims to map the global research landscape on the application of Trichoderma in controlling Fusarium wilt through bibliometric analysis and literature review. Data were retrieved from the Scopus database (2015–2025) using selected keywords and analyzed with VOSviewer and Biblioshiny to identify publication trends, author collaborations, institutions, countries, and keyword clusters. The results indicate a marked increase in publications since 2020, with major contributions originating from India, China, and Egypt. The evolution of keywords indicates a shift in research focus from basic mechanistic studies to practical field applications. The literature review confirms the effectiveness of Trichoderma in suppressing disease and enhancing the productivity of agricultural commodities, although challenges persist in formulation, variability, and regulatory frameworks. These findings provide an important scientific basis for guiding future research, strengthening international collaboration, and supporting sustainable agricultural strategies that utilize biological control agents.

References

Abdenaceur, R., Farida, B. tihar, Mourad, D., Rima, H., Zahia, O., & Fatma, S. H. (2022). Effective biofertilizer Trichoderma spp. isolates with enzymatic activity and metabolites enhancing plant growth. International Microbiology, 25(4), 817–829. https://doi.org/10.1007/s10123-022-00263-8

Cao, X., Liang, J., Wu, Z., Zhang, M., Li, H., Liu, T., Yue, W., Wang, Y., Jiang, L., Wang, G., Zhao, P., Zhou, Y., Chen, X., Sui, J., Hou, D., Song, X., & Zhang, X. (2025). Biocontrol mechanisms of Trichoderma longibrachiatum SMF2 against lanzhou lily wilt disease caused by Fusarium oxysporum and fusarium solani. Horticulturae, 11(6), 1–19. https://doi.org/10.3390/horticulturae11060660

Chen, S. C., Zhao, H. J., Wang, Z. H., Zheng, C. X., Zhao, P. Y., Guan, Z. H., Qin, H. Y., Liu, A. R., Lin, X. M., & Ahammed, G. J. (2017). Trichoderma harzianum-induced resistance against Fusarium oxysporum involves regulation of nuclear DNA content, cell viability and cell cycle-related genes expression in cucumber roots. European Journal of Plant Pathology, 147(1), 43–53. https://doi.org/10.1007/s10658-016-0978-7

Correa-Delgado, R., Brito-López, P., Cardoza, R. E., Jaizme Vega, M. C., Laich, F., & Gutiérrez, S. (2024). Biocontrol potential of a native trichoderma collection against Fusarium oxysporum f. sp. cubense Subtropical Race 4. Agriculture (Switzerland), 14(11). https://doi.org/10.3390/agriculture14112016

Cui, H., Cheng, Q., Jing, T., Chen, Y., Li, X., Zhang, M., Qi, D., Feng, J., Vafadar, F., Wei, Y., Li, K., Zhao, Y., Zhou, D., & Xie, J. (2025). Trichoderma virens XZ11-1 producing siderophores inhibits the infection of Fusarium oxysporum and promotes plant growth in banana plants. Microbial Cell Factories, 24(1). https://doi.org/10.1186/s12934-024-02626-4

El Komy, M. H., Saleh, A. A., Eranthodi, A., & Molan, Y. Y. (2015). Characterization of novel Trichoderma asperellum isolates to select effective biocontrol agents against tomato fusarium Wilt. The Plant Pathology Journal, 31(1), 50–60. https://doi.org/10.5423/PPJ.OA.09.2014.0087

Hadiwiyono, H., Sari, K., & Poromarto, S. H. (2020). Yields Losses Caused by Basal Plate Rot (Fusarium oxysporum f.sp. cepae) in some shallot varieties. Caraka Tani: Journal of Sustainable Agriculture, 35(2), 250. https://doi.org/10.20961/carakatani.v35i2.26916

Hernández, G., Ponce de la Cal, A., Louis, Y., Baró Robaina, Y., Coll, Y., Spengler, I., & Mirabal-Gallardo, Y. (2024). Identification of secondary metabolites by UHPLC-ESI-HRMS/MS in antifungal strain Trichoderma harzianum (LBAT-53). Journal of Fungi, 10(8), 547. https://doi.org/10.3390/jof10080547

Hugar, A., & Nayaka, S. (2025). Trichoderma harzianum isolate AKH-5 enhances defense response in Cajanus cajan (L.) millsp. against Fusarium oxysporum f. sp. udum and promotes plant growth. Microbe (Netherlands), 8, 100454. https://doi.org/10.1016/j.microb.2025.100454

Javeria, S., Kumar, A., Kharkwal, A. C., Varma, A., Srinivasa, N., & Sharma, P. (2020). Evaluation of rhizospheric Trichoderma species strains for producing cell wall-degrading and defense related enzymes in response to Fusarium oxysporum f. sp. lentis. Indian Phytopathology, 73(3), 461–467. https://doi.org/10.1007/s42360-020-00262-7

Jogaiah, S., Abdelrahman, M., Tran, L. P., & Ito, S. (2018). Different mechanisms of Trichoderma virens ‐mediated resistance in tomato against Fusarium wilt involve the jasmonic and salicylic acid pathways. Molecular Plant Pathology, 19(4), 870–882. https://doi.org/10.1111/mpp.12571

Joo, J. H., & Hussein, K. A. (2022). Biological control and plant growth promotion properties of volatile organic compound-producing antagonistic Trichoderma spp. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.897668

Kumari, R., Kumar, V., Arukha, A. P., Rabbee, M. F., Ameen, F., & Koul, B. (2024). Screening of the Biocontrol Efficacy of Potent Trichoderma Strains against Fusarium oxysporum f.sp. ciceri and Scelrotium rolfsii Causing Wilt and Collar Rot in Chickpea. Microorganisms, 12(7), 1–18. https://doi.org/10.3390/microorganisms12071280

Kumari, R., Kumar, V., Koul, B., Abul Farah, M., & Mishra, A. K. (2025). Synergistic effects of Trichoderma and biochar on the biocontrol of two soil-borne phytopathogens in chickpeas. Frontiers in Microbiology, 16. https://doi.org/10.3389/fmicb.2025.1583114

Lal, D., Dev, D., Kumari, S., Pandey, S., Aparna, Sharma, N., Nandni, S., Jha, R. K., & Singh, A. (2024). Fusarium wilt pandemic: current understanding and molecular perspectives. Functional & Integrative Genomics, 24(2), 41. https://doi.org/10.1007/s10142-024-01319-w

LI, M., MA, G. shu, LIAN, H., SU, X. lin, TIAN, Y., HUANG, W. kun, MEI, J., & JIANG, X. liang. (2019). The effects of Trichoderma on preventing cucumber fusarium wilt and regulating cucumber physiology. Journal of Integrative Agriculture, 18(3), 607–617. https://doi.org/10.1016/S2095-3119(18)62057-X

Lombardi, N., Vitale, S., Turrà, D., Reverberi, M., Fanelli, C., Vinale, F., Marra, R., Ruocco, M., Pascale, A., D’Errico, G., Woo, S. L., & Lorito, M. (2018). Root exudates of stressed plants stimulate and attract trichoderma soil fungi. Molecular Plant-Microbe Interactions®, 31(10), 982–994. https://doi.org/10.1094/MPMI-12-17-0310-R

Mishra, R. K., Pandey, S., Hazra, K. K., Mishra, M., Satheesh Naik, S. J., Bohra, A., Parihar, A. K., Rathore, U. S., Naimuddin, Kumar, K., Singh, B., & Singh, N. P. (2023). Biocontrol efficacy and induced defense mechanisms of indigenous Trichoderma strains against Fusarium wilt [F. udum (Butler)] in pigeonpea. Physiological and Molecular Plant Pathology, 127, 102122. https://doi.org/10.1016/j.pmpp.2023.102122

Muhorakeye, M. C., Namikoye, E. S., Khamis, F. M., Wanjohi, W., & Akutse, K. S. (2024). Biostimulant and antagonistic potential of endophytic fungi against fusarium wilt pathogen of tomato Fusarium oxysporum f. sp. lycopersici. Scientific Reports, 14(1), 1–17. https://doi.org/10.1038/s41598-024-66101-1

Nofal, A. M., El-Rahman, M. A., Abdelghany, T. M., & Abd El-Mongy, M. (2021). Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato. Egyptian Journal of Biological Pest Control, 31(1), 1–8. https://doi.org/10.1186/s41938-021-00450-1

Pradhan, D. A., Bagagoni, P., & Makandar, R. (2023). Assessing rhizosphere Trichoderma asperellum strains for root colonizing and antagonistic competencies against Fusarium wilt through molecular and biochemical responses in castor. Biological Control, 184, 105280. https://doi.org/10.1016/j.biocontrol.2023.105280

Rajani, P., Rajasekaran, C., Vasanthakumari, M. M., Olsson, S. B., Ravikanth, G., & Uma Shaanker, R. (2021). Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds. Microbiological Research, 242, 126595. https://doi.org/10.1016/j.micres.2020.126595

Rauf, A., Subhani, M. N., Siddique, M., Shahid, H., Chattha, M. B., Alrefaei, A. F., Hasan Naqvi, S. A., Ali, H., & Lucas, R. S. (2024). Cultivating a greener future: exploiting trichoderma derived secondary metabolites for fusarium wilt management in peas. Heliyon, 10(7), e29031. https://doi.org/10.1016/j.heliyon.2024.e29031

Saravanakumar, K., Yu, C., Dou, K., Wang, M., Li, Y., & Chen, J. (2016). Synergistic effect of Trichoderma-derived antifungal metabolites and cell wall degrading enzymes on enhanced biocontrol of Fusarium oxysporum f. sp. cucumerinum. Biological Control, 94, 37–46. https://doi.org/10.1016/j.biocontrol.2015.12.001

Singh, P., Singh, J., Ray, S., Rajput, R. S., Vaishnav, A., Singh, R. K., & Singh, H. B. (2020). Seed biopriming with antagonistic microbes and ascorbic acid induce resistance in tomato against Fusarium wilt. Microbiological Research, 237, 126482. https://doi.org/10.1016/j.micres.2020.126482

Srinivas, C., Nirmala Devi, D., Narasimha Murthy, K., Mohan, C. D., Lakshmeesha, T. R., Singh, B., Kalagatur, N. K., Niranjana, S. R., Hashem, A., Alqarawi, A. A., Tabassum, B., Abd_Allah, E. F., Chandra Nayaka, S., & Srivastava, R. K. (2019). Fusarium oxysporum f. sp. lycopersici causal agent of vascular wilt disease of tomato: Biology to diversity– A review. Saudi Journal of Biological Sciences, 26(7), 1315–1324. https://doi.org/10.1016/j.sjbs.2019.06.002

Wu, Q., Sun, R., Ni, M., Yu, J., Li, Y., Yu, C., Dou, K., Ren, J., & Chen, J. (2017). Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy. PLOS ONE, 12(6), e0179957. https://doi.org/10.1371/journal.pone.0179957

Yao, X., Guo, H., Zhang, K., Zhao, M., Ruan, J., & Chen, J. (2023). Trichoderma and its role in biological control of plant fungal and nematode disease. Frontiers in Microbiology, 14, 1–15. https://doi.org/10.3389/fmicb.2023.1160551

You, J., Li, G., Li, C., Zhu, L., Yang, H., Song, R., & Gu, W. (2022). Biological Control and Plant Growth Promotion by Volatile Organic Compounds of Trichoderma koningiopsis T-51. Journal of Fungi, 8(2), 131. https://doi.org/10.3390/jof8020131

Zawawy, N. A. E., El-Esawi, M. A., Attia, N., & Mahmoud, Y. A. G. (2025). Biocontrol potential of endophytic Trichoderma harzianum AUMC 14897 against Fusarium seedling blight disease in oat. BMC Plant Biology, 25(1). https://doi.org/10.1186/s12870-025-06517-7

Zhang, F., Liu, C., Wang, Y., Dou, K., Chen, F., Pang, L., Kong, X., Shang, C., & Li, Y. (2020). Biological characteristic and biocontrol mechanism of Trichoderma harzianum T-A66 against bitter gourd wilt caused by Fusarium oxysporum. Journal of Plant Pathology, 102(4), 1107–1120. https://doi.org/10.1007/s42161-020-00573-8

Zhou, W., Li, M., & Achal, V. (2025). A comprehensive review on environmental and human health impacts of chemical pesticide usage. Emerging Contaminants, 11(1), 100410. https://doi.org/10.1016/j.emcon.2024.100410

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Published

27-02-2026

How to Cite

Putra, A. M. (2026). Trichoderma-Based Biocontrol Strategies Against Fusarium Wilt: A Bibliometric Analysis and literature Review. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 11(1), 94–108. https://doi.org/10.24002/biota.v11i1.12938