Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes

Authors

  • Agus Hery Susanto
  • Suhestri Suryaningsih

DOI:

https://doi.org/10.24002/biota.v11i3.2539

Keywords:

sand goby, isozyme, biochemical-genetic variation, horizontal electrophoresis

Abstract

Penjalin water reservoir in Brebes Regency, Central Java, is one of the habitats of the sand goby. A study on its genetic diversity using approaches of isozyme analysis was needed to support domestication of the fish in this area. This study was aimed at the biochemical-genetic variation of sand goby population in Penjalin water reservoir based on esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), aspartate amino-transferase (AAT), and acid phosphatase (ACP) polymorphisms. Visualization of the isozymes was carried out employing horizontal electrophoretic technique with potato starch gel and buffer system of L-histidin monohydrate. Of the five isozymes, ACP was not well-visualized in all samples tested while the remaining four showed no polymorphisms. It could be concluded that there is no biochemical-genetic variation of sand goby population in Penjalin water reservoir based on isozymes of EST, PER, MDH, and AAT.

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Published

08-10-2019

How to Cite

Susanto, A. H., & Suryaningsih, S. (2019). Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 11(3), 136–141. https://doi.org/10.24002/biota.v11i3.2539