Optimasi Produksi Poli-β-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10

Authors

  • Nur Arfa Yanti
  • Sebastian Margino
  • Langkah Sembiring

DOI:

https://doi.org/10.24002/biota.v15i3.2587

Keywords:

Poly-β-hydroxybutyrate (PHB) production, Bacillus, sago starch, optimization

Abstract

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-β-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.

Downloads

Published

15-10-2019

How to Cite

Yanti, N. A., Margino, S., & Sembiring, L. (2019). Optimasi Produksi Poli-β-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 15(3), 331–339. https://doi.org/10.24002/biota.v15i3.2587

Issue

Vol 15, No 3 (2010): October 2010

Section

Articles

License

Authors who publish with Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati agree to the following terms:

  • Authors retain copyright and grant the Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati right of first publication. Licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, and as long as Author is not used for commercial purposes.