Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12

Catur Retnaningdyah, Suharjono Suharjono, Agoes Soegianto, Bambang Irawan


The main objective of this research was to calculate the carrying capacity and growth rate of
isolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphate
concentrations in the B-12 selective medium. Research was conducted in the laboratory with
pure experiments using completely randomized factorial design with factors of nitrate
concentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12
medium. Repetition of the study was conducted three times at the same time. Microcystis
population abundance which was counted every day until the stationary phase (day +30) was
used to calculate the rate of growth (β) and maximum abundance of Microcystis can be
supported by each medium treatment (γ). The results showed that the growth rate of Microcystis
was not significantly influenced by levels of phosphate in the medium but significantly positively
correlated with increasing nitrate concentration in the medium. Carrying capacity or the
maximum abundance (γ) of Microcystis was influenced by the combination of nitrate and
phosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined with
nitrate 8−64 ppm could support the highest abundance of Microcystis.


Microcystis, B-12 medium, nitrate and phosphate, Sutami Reservoir

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