Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal)

Authors

  • Tatty Yuniarti
  • Tati Nurhayati
  • Agoes Mardiono Jacoeb

DOI:

https://doi.org/10.24002/biota.v15i3.2593

Keywords:

Collagenase, purification, intestines, milkfish

Abstract

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,
ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gel
filtration on a Sephadex G-100 column. The purification and yields were 114.731 fold and
1.26% when compared to those in the starting-crude extract. The molecular mass of milkfish
serine collagenase was estimated to be 14.63 kDa and 27.46 kDa.

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Published

15-10-2019

How to Cite

Yuniarti, T., Nurhayati, T., & Jacoeb, A. M. (2019). Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal). Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 15(3), 379–384. https://doi.org/10.24002/biota.v15i3.2593

Issue

Vol 15, No 3 (2010): October 2010

Section

Articles

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