Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal)

Tatty Yuniarti, Tati Nurhayati, Agoes Mardiono Jacoeb

Abstract


Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,
ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gel
filtration on a Sephadex G-100 column. The purification and yields were 114.731 fold and
1.26% when compared to those in the starting-crude extract. The molecular mass of milkfish
serine collagenase was estimated to be 14.63 kDa and 27.46 kDa.


Keywords


Collagenase, purification, intestines, milkfish

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DOI: https://doi.org/10.24002/biota.v15i3.2593

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