Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans

Authors

  • Joko Sulistyo
  • Yati Sudaryati Soeka

DOI:

https://doi.org/10.24002/biota.v14i1.2629

Keywords:

CGT-ase, Bacillus macerans, transglycosylation, genistin, antimutagenesis

Abstract

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.

Downloads

Published

19-02-2009

How to Cite

Sulistyo, J., & Soeka, Y. S. (2009). Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 14(1), 28–36. https://doi.org/10.24002/biota.v14i1.2629

Issue

Vol 14, No 1 (2009): February 2009

Section

Articles

License

Authors who publish with Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati agree to the following terms:

  • Authors retain copyright and grant the Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati right of first publication. Licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, and as long as Author is not used for commercial purposes.