Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit

Authors

  • I Ketut Puja
  • I Nyoman Sulabda

DOI:

https://doi.org/10.24002/biota.v14i1.2632

Keywords:

genetics characteristic, Gembrong Goat, DNA mikrosatellite

Abstract

The present study was undertaken with primary objective to characterize of Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA Genome was isolated from hairs. Nine microsatellite were amflified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. Flourescent signals from the dye-labeled microsatellite were detecting using STRand software. The result show that all markers (INRA005, INRA063, INRA023, ILSTS19, ILSTS87, SRCRSP8, MAF65, OarFCB20, and McM547) successfully amplified in Gembrong goat microsatellite loci. The number of alleles per locus ranged from 1 (INRA005) to 4 (ILSTS87). All the microsatellites investigated were found to be highly polymorphic, except INRA063. In total, 23 alleles were observed for the 9 microsatellite loci. The allele sizes ranged from 99 bp (OarFCB20) to 240bp (SRCRSP8). The study can be extended to include large many microsatellites in different chromosome location to validate the results.

Downloads

Published

19-02-2009

How to Cite

Puja, I. K., & Sulabda, I. N. (2009). Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 14(1), 45–49. https://doi.org/10.24002/biota.v14i1.2632

Issue

Vol 14, No 1 (2009): February 2009

Section

Articles

License

Authors who publish with Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati agree to the following terms:

  • Authors retain copyright and grant the Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati right of first publication. Licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, and as long as Author is not used for commercial purposes.