Induksi Mutasi Curcuma zedoaria (Christm) Roscoe dengan Irradiasi Sinar Gamma
DOI:
https://doi.org/10.24002/biota.v14i2.2686Keywords:
Curcuma zedoaria, random amplified polymorphic DNA, genetic variation, gamma irradiationAbstract
Curcuma zedoaria (Zingiberaceae) is one of Curcuma spp. used as stomachic,
carminative, haematic and styptic medicines. Dried rhizomes of C. zedoaria contain
essential oil and curcuminoids. Induced mutation of this species would provide and
help plant breeders to improve and select better quality of the plant. The present
study was carried out to determine LD-50% of gamma irradiation of C. zedoaria, to
induce genetic variation using gamma irradiation, and to assess genetic diversity
among mutant genotypes using random amplified polymorphic DNA (RAPD)
markers. The LD-50% of gamma irradiation was achieved at 813.32 rad. Chlorophyll
mutations were recorded at vM1 and vM2. Result of genetic analysis showed that
RAPD profiles were different from that of the control, and genetic dissimilarities
among mutant genotypes were higher than that of the control.
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