Karakterisasi, Pengaruh Sumber Nitrogen dan Karbon terhadap Produktivitas Enzim Lipase Rhizopus microsporus var oligosporus UICC 550

Wibowo Mangunwardoyo, Yuyun Lusini, Indrawati Gandjar

Abstract


Effect of nitrogen and carbon source on the production of extracellular lipase by R.
microsporus var. oligosporus UICC 550 was studied. The enzyme activity was also
characterized in terms of temperature, pH, stability at room temperature, and effect
of divalent ion. The amount of 2.5% (w/v) of olive oil as carbon sources and 5% (w/v)
peptone as nitrogen sources were the optimum for production of lipase enzyme.
Partial purifications using ammonium sulfate followed by dialysis showed that at pH
6.5 and temperature 35oC was the optimum condition, respectively. The stability
(remaining up to 80% of the optimum enzyme) was recorded at pH 6.5 after 24 hours
incubation at room temperature. The optimum activity remained 40% of the after one
hour incubation at 35oC. Divalent ions concentration at 1 mM, Zn2+, Cu2+, Mg2+ and
Fe2+ inhibited the lipolytic activity.


Keywords


amonium sulphate, olive oil, peptone, Rhizopus microsporus var oligosporus UICC 550, pH and temperature optimum

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DOI: https://doi.org/10.24002/biota.v14i2.2689

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