Sistematik Filogenetik Isolat-isolat Kapang Indigenous Indonesia sebagai Entomopatogen Kutu Sisik (Lepidoshapes beckii Newman) Hama Tanaman Jeruk

Authors

  • Suharjono Suharjono
  • Agung P.W. Marhendra
  • Anang Triwiratno
  • Susi Wuryantini
  • Lina Oktavia R

DOI:

https://doi.org/10.24002/biota.v15i2.2708

Keywords:

Entomopathogen, mould, purple scale insect, phylogenetic, 28S rDNA

Abstract

Citrus fruits constitute a basic agricultural product in many countries. They are a source of
national income, empowering labor market in rural zones and those surrounding urban areas,
and in addition to being an important part of the diet. Insects are common problems to planting
of citrus. Purple Scale, Lepidosaphes beckii (Newman) is one of the destructive insect pests of
citrus worldwide. The control of purple scale insects in citrus utilizes native natural enemies
including pathogens. Most known species of mould are pathogens of purple scale insect of citrus.
The objective of this research was to study phylogenetic systematics of Indonesian indigenous
mould isolates as pathogen on purple scale insect (Lepidoshapes beckii Newman) pest of citrus
plants. Seven mould isolates derived from Purworejo, Brastagi, and Poncokusumo were showed
pathogenic on purple scale insect. The fungal isolates were cultured on PDA. The DNA of fungal
material was isolated with Kit Prepman Ultra (Applied Biosystems). Nuclear ribosomal Large
Subunit 28S rDNA (LSU) was amplified using primers ITS1 and ITS4. Products of PCR were
purified and sequenced. Based on the 28S rDNA sequence, the result was showed that isolate of
PW ASG D4 was related to Gibberella moniliformis dx-12, PC ORG D4 was related to Gibberella sp.
1893, PW ASG D2 was related to Gibberella moniliformis BZ070101, PW LB D2 was related to
Gibberella moniliformis 52, BRS D22 was related to Fusarium oxysporum DC-1-67, PWRJ I D4 was
related to Fusarium culmorum SQ070108, and BRS D23 was related to Aschersonia sp. Ag-11.

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Published

2011-06-01

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Articles