Karakterisasi Protease Ekstraseluler Clostridium spp. T11-3

Authors

  • Loli Natalia
  • Lily Nathalia
  • Anja Meryandini

DOI:

https://doi.org/10.24002/biota.v11i1.2822

Keywords:

Clostridium spp T11-3, protease Exstracellular, divalent ion

Abstract

Protease is one of the leather commercial enzymes which is widely used such in food processing, medicine and leather industry. Clostridium sp T11-3 was isolated from Tiu Jeruk River in Nusa Tenggara Barat. Sequence analysis of 16S rRNA indicated that Clostridium spp T11-3 was closely related to C. bifermentans. This isolate produced maximum protease activity after 18 hours of cultivation in liquid media. Protease of Clostridium spp 11-3 displayed maximum activity at pH 5 and 60oC with casein as substrate. In the presence of 1 mM divalent ion Mg2+ the enzym activity increased to 141 %, while others ion divalent (Ca2+, Zn2+, Cu2+, Fe2+, and Co2+) inhibited protease activity.

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Published

03-11-2019

How to Cite

Natalia, L., Nathalia, L., & Meryandini, A. (2019). Karakterisasi Protease Ekstraseluler Clostridium spp. T11-3. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 11(1), 47–51. https://doi.org/10.24002/biota.v11i1.2822

Issue

Vol 11, No 1 (2006): February 2006

Section

Articles

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