Konstruksi Open Reding Frames (ORF) Artifisial Berukuran 798-bp yang Menyandi Protein dengan Urutan Asam Amino Acak

Authors

  • Irfan Dwidya Prijambada
  • Khotibul Umam Al-Awally
  • Muhammad Saifur Rohman
  • Wayan Artama

DOI:

https://doi.org/10.24002/biota.v9i1.2828

Keywords:

ORF buatan, urutan asam amino acak

Abstract

Penyusunan pustaka dari open reading frames (ORF) buatan yang tersusun atas 798 bp (pasangan basa), 576  di antaranya tersusun secara acak, yang mampu menyandi 266 asam amino telah berhasil dilakukan. Dalam upaya penyusunan tersebut diperoleh 32 transforman,  lima di antaranya membawa ORF buatan. Dari kelima transforman yang membawa ORF buatan tersebut, hanya satu transforman yang mampu berekspresi dan menyandi suatu protein. Protein yang dihasilkan memiliki ukuran 17 kDa, berukuran lebih kecil daripada ukuran yang diharapkan yaitu 29 kDa.

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Published

03-11-2019

How to Cite

Prijambada, I. D., Al-Awally, K. U., Rohman, M. S., & Artama, W. (2019). Konstruksi Open Reding Frames (ORF) Artifisial Berukuran 798-bp yang Menyandi Protein dengan Urutan Asam Amino Acak. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 9(1), 37–40. https://doi.org/10.24002/biota.v9i1.2828

Issue

Vol 9, No 1 (2004): February 2004

Section

Articles

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