Genotipe Single Nucleotide Polimorphism (SNP) Gen PLA2G10 T512C dan T-123/IN1C pada Penderita Angina Pektoris di Pusat Jantung Nasional Harapan Kita Jakarta, Indonesia
DOI:
https://doi.org/10.24002/biota.v5i3.3345Keywords:
pla2g10, snp id, t512c, t-123/in1c, angina pectoris, polymorphismAbstract
Angina pektoris merupakan salah satu gejala awal yang menjadi penanda masalah kardiovaskuler berat seperti penyakit jantung koroner. Sementara gen PLA2G10 dipercaya memiliki peran, baik dalam progresi aterosklerotik yang menyebabkan deposit plak dan penyumbatan pada arteri koroner, atau justru sebaliknya yaitu antiaterogenik. Polimorfisme PLA2G10 pada titik SNP T512C (rs36072688) dan T-123/in1C (rs4003232) mampu mengubah level ekspresi gen PLA2G10 sebagai gen pengode enzim sPLA2-GX. Penelitian ini bertujuan untuk mengetahui genotip dari PLA2G10 pada titik SNP T512C (rs36072688) and T-123/in1C (rs4003232) para penderita angina pektoris dan mengkaji apakah penyakit disebabkan karena mutasi. Sampel penelitian berupa peripheral blood mononuclear cell koleksi Divisi Litbang Rumah Sakit Jantung dan Pembuluh Darah Harapan Kita. Sampel berjumlah 113, berasal dari penderita angina pektoris dengan dan tanpa plak pada arteri koroner. Hasil penelitian menunjukkan bahwa gen PLA2G10 pada titik SNP T512C (rs36072688) memiliki genotip homozigot wildtype (TT) dan T-123/In1C (rs4003232) memiliki genotip heterozigot (TC) melalui metode TaqMan® SNP Genotyping Assay, dan tidak ada polimorfisme yang ditemukan pada kedua grup sampel tersebut.
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