Perbandingan Metode Isolasi DNA sebagai Templat PCR untuk Identifikasi Jenis Kelamin Cerek Jawa (Charadriius javanicus) secara Molekuler Menggunakan Primer 2550F/2718R

Authors

DOI:

https://doi.org/10.24002/biota.v6i2.3444

Keywords:

Javan Plover, Molecular Sexing, 2550F/2718R, Extraction kit, Alkaline lysis, Direct PCR

Abstract

Dalam studi molekuler, ekstraksi dapat memakan waktu dan biaya saat diterapkan dalam penelitian skala besar, sehingga beberapa studi mengkaji tingkat keberhasilan amplifikasi menggunakan metode isolasi DNA alternatif (tanpa ekstraksi). Penelitian ini bertujuan untuk mengetahui metode isolasi DNA paling efektif untuk molecular sexing Cerek Jawa. Penelitian ini menggunakan 5 sampel darah kertas saring, dan 8 sampel darah EDTA yang diisolasi dengan metode ekstraksi kit, alkalin lisis dan PCR langsung. Molecular sexing dilakukan dengan primer 2550F/2718R. Hasil penelitian menunjukan bahwa DNA pada sampel darah kertas saring dan EDTA, baik metode ektraksi kit, alkalin lisis dan PCR langsung, berhasil diamplifikasi dengan primer 2550F/2718R yang menghasilkan pita CHD1-Z dengan ukuran 621 bp dan pita CHD1-W dengan ukuran 459 bp. Penelitian ini menunjukan bahwa ekstraksi DNA dengan kit menghasilkan DNA template yang paling efektif untuk molecular sexing Cerek Jawa.

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Published

10-06-2021

How to Cite

Pratomo, Y. W., Zahida, F., & Yuda, P. (2021). Perbandingan Metode Isolasi DNA sebagai Templat PCR untuk Identifikasi Jenis Kelamin Cerek Jawa (Charadriius javanicus) secara Molekuler Menggunakan Primer 2550F/2718R. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 6(2), 78–86. https://doi.org/10.24002/biota.v6i2.3444

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Vol 6, No 2 (2021): June 2021

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Copyright (c) 2021 Yulius Wahyu Pratomo, Felicia Zahida, Pramana Yuda

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