Aktivitas Antibakteri Rimpang Meistera chinensis terhadap Bakteri Staphylococcus aureus ATCC 25023 dan Eschericia coli ATCC 35218 Secara Difusi Agar
DOI:
https://doi.org/10.24002/biota.v8i1.5651Keywords:
antibakteri, Escherichia coli, rimpang Meistera chinensis, Staphylococcus aureus, ZingiberaceaeAbstract
Meistera chinensis merupakan generasi baru famili Zingiberaceae yang tumbuh di Sulawesi Tenggara. Ekstrak buah mengandung metabolit sekunder seperti flavonoid, terpenoid, alkaloid, steroid, dan tanin yang memiliki efektivitas sebagai antioksidan dan antibakteri. Namun, belum ada laporan tentang antibakteri pada bagian rimpang. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder dan aktivitas antibakteri ekstrak etanol rimpang M. chinensis. Serbuk kering rimpang M. chinensis diekstraksi dengan maserasi menggunakan pelarut etanol 96%. Ekstrak dipekatkan menggunakan rotary vacuum evaporator. Metode analisis aktivitas antibakteri menggunakan difusi agar dengan konsentrasi ekstrak 10%, 20%, 30%, dan kontrol positif (Ciprofloxacin 30µg) terhadap Staphylococcus aureus ACTT 25023 dan Escherichia coli ACTT 35218 selama 24 jam. Hasil penelitian menunjukkan bahwa ekstrak etanol rimpang M. chinensis mengandung flavonoid, saponin, alkaloid, steroid, dan fenol, Ekstrak terbukti menghambat pertumbuhan E. coli dengan diameter zona hambat rata-rata secara berturut-turut pada konsentrasi 10%, 20%, dan 30% sebesar 6,08±1,79; 8,16±0,11 dan 10,57±1,34 mm. Pada S. aureus, zona hambat masing-masing konsentrasi 10%, 20%, dan 30% sebesar 5,02±0,79; 6,01±0,69; 8,03±0,76. Analisis One Way ANOVA menunjukkan perbedaan aktivitas antibakteri yang signifikan antar konsentrasi ekstrak pada E. coli dan S. aureus. Dapat disimpulkan rimpang M. chinensis memiliki aktivitas sebagai antibakteri.
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Copyright (c) 2023 Karmilah, Reymon, Nur Saadah Daud, Esti Badia, Agung Wibawa Mahatva Yodha, Muh. Azdar Setiawan, Selfyana Austin Tee, Musdalipah
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