Analisis In Silico Naringenin dari Umbi Akar Batu (Gerrardanthus macrorhizus Harv.ex Benth. & Hook.f.) sebagai Antitusif terhadap Reseptor N-methyl-D-aspartate

Authors

  • Maria Angelina Genere Koban Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Malang
  • Sri Rahayu Lestari Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Malang
  • Frida Kunti Setiowati Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Malang

DOI:

https://doi.org/10.24002/biota.v7i3.5912

Keywords:

antitusif, dekstrometorfan, in silico, N-metil-D-aspartat, umbi akar batu

Abstract

Batuk  merupakan refleks pertahanan tubuh alami yang membersihkan saluran pernapasan dari benda asing. Dekstrometorfan merupakan obat antitusif yang bersifat antagonis non-competitive chanel blocker terhadap reseptor N-Methyl-D-aspartate yang mampu menekan batuk di pusat ambang batuk. Penelitian ini bertujuan untuk menganalisis potensi naringenin sebagai antitusif berdasarkan nilai afinitas pengikatannya dengan reseptor NMDA, sifat farmakokinetik, potensi biooral dan toksisitas melalui pendekatan in silico dengan teknik molecular docking. Hasil penelitian menunjukkan, bahwa dekstrometorfan dan naringenin berikatan di residu asam amino yang sama dengan NMDA pada MET A: 145, ILE A: 85, ALA B: 888 dengan ikatan hidrofobik, SER A: 81 dengan ikatan hidrogen pada dekstrometorfan dan ikatan unfavorabel donor-donor pada naringenin. Nilai binding affinitas naringenin -6,7 kkal/mol lebih kecil dibandingkan dengan dekstrometorfan -6,4 kkal/mol, hal ini menunjukkan bahwa naringenin berpotensi sebagai antitusif yang hampir sama dengan dekstrometorfan. Hasil uji toksisitas, menunjukan bahwa naringenin dan dekstrometorfan dapat dikonsumsi secara oral dan tidak berbahaya bagi kesehatan. Analisis potensi bioral dan uji farmakokinetik membuktikan bahwa naringenin mampu diabsorbsi, didistribusi, dimetabolisme dan diekskresi, sehingga berpotensi sebagai kandidat obat antitusif. 

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30-10-2022

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