Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense

Authors

  • Vania Agustinus Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana Yogyakarta
  • Ratih Restiani Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana Yogyakarta
  • Aniek Prasetyaningsih Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana Yogyakarta

DOI:

https://doi.org/10.24002/biota.v9i2.7728

Keywords:

Air kelapa, BAP, Dendrobium hibrida, regenerasi protokorm, Vacin and Went

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.

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Published

14-06-2024

How to Cite

Agustinus, V. ., Restiani, R., & Prasetyaningsih, A. . (2024). Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense . Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 9(2), 199–209. https://doi.org/10.24002/biota.v9i2.7728

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Vol 9, No 2 (2024): June 2024

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Copyright (c) 2024 Vania Agustinus, Ratih Restiani, Aniek Prasetyaningsih

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